HPSE Antibody

• Gene Name:

  Heparanase

• Gene Aliases:

  HPA, HPA1, HPR1, HSE1, HPSE1

• Gene ID:

  10855

• Accession Number:

  NP_001092010.1NP_001159970.1NP_001186759.1NP_006656.2

• Reactivity:

  Human

• Immunogen:

  Mab HP3/17 is a monoclonal antibody raised against a polypeptide from the 50 kDa subunit of Heparanase

• Target:

  Heparanase is an endo-b-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3) . Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5) . Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6) . Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8) .

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Applications:

  WB, IHC

• Purification:

  Protein G affinity purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Each vial contains 50 ug in 12.5 ul respectively, of 0.22 micron filtered solution of 20 mM Sodium Phosphate; 150 mM NaCl; pH 7.2, containing 0.001% Thimerosal.

• Reconstitution:

  Store at 4C. Stable for six months from the date of shipment. For extended storage, freeze in working aliquots at -20C. Avoid repeated freeze-thaw cycles.

• Notes:

  Formerly GWB-3CA9D3

• Fragment:

  Mouse IgG2Bk.

• Specificity:

  HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor of human or mouse Heparanase by Western blotting and immunohistochemistry.Recommended dilution range for Western blot analysis: 1:4000. Recommended dilution range for immunohistochemistry: 1:40.

• NCBI Gene Symbol:

  HPSE

• Host or Source:

  Mouse

• Protein Name:

  Heparanase

• Gene Name URL:

  HPSE

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001098540.2NM_001166498.2NM_001199830.1NM_006665.5