ACTA1 Antibody

• Gene Aliases:

  ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, ASMA, NEM3

• Gene ID:

  58

• Accession Number:

  NP_001091.1

• Reactivity:

  Rabbit, Goat, Rat, Pig, Human

• Immunogen:

  Peptide comprising the N-Terminus nonapeptide of alpha-skeletal actin with an acetylated N-Terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue

• Target:

  Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity.

• Clonality:

  Monoclonal

• Clone:

  3B3

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  ELISA, IHC, IHC-F, IHC-P, WB

• Purification:

  Protein G purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid// PBS containing 0.09% sodium azide

• Buffer:

  PBS, 0.09% Sodium Azide

• Reconstitution:

  Short term: store at 4C. Long term: store at -20C. Avoid freeze-thaw cycles.

• Fragment:

  IgG1

• Specificity:

  Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore the antibody cross-reacts with many other species.

• NCBI Gene Symbol:

  ACTA1

• Host or Source:

  Mouse

• Gene Name URL:

  ACTA1

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001100