CDKN2A Antibody

• Gene Aliases :

  , INK4A, Multiple tumor suppressor 1, MTS-1, MTS1, p16-INK4A, p16 INK4A, p16INK4A, CDKN2A, cyclin-dependent kinase inhibitor 2A

• Accession Number :

  NP_000068.1

• Reactivity :

  Mouse, Rat, Human

• Immunogen :

  Full length recombinant p16INK4a NP_000068

• Target :

  Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.

• Clonality :

  Monoclonal

• Clone :

  1E12E10

• Conjugation :

  Unconjugated

• Type :

  Monoclonal Antibody

• Applications :

  ELISA, IHC, IHC-P, WB

• Purification :

  Affinity purified from ascites

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Liquid// PBS with 0.1% sodium azide and 1% BSA

• Buffer :

  PBS, 0.1% Sodium Azide, 1% BSA

• Reconstitution :

  Short term: store at 4C. Long term: store at -20C. Avoid freeze-thaw cycles.

• Fragment :

  IgG1

• Specificity :

  Targets P16. Shows reactivity with Human, Mouse, and Rat samples.

• NCBI Gene Symbol :

  CDKN2A

• Host or Source :

  Mouse

• Gene Name URL :

  CDKN2A

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_000077