ITGAM Antibody

• Gene Aliases:

  ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

• Gene ID:

  3684

• Accession Number:

  NP_000623.2

• Reactivity:

  Rabbit, Mouse, Human

• Immunogen:

  T cell enriched splenocytes from B10 mice

• Clonality:

  Monoclonal

• Clone:

  M1/70.15

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  FC, IF, IHC, IHC-F, IHC-P, IP

• Purification:

  Ion exchange chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid

• Buffer:

  PBS, 0.09% Sodium Azide

• Reconstitution:

  Store at 4C or at -20C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

• Fragment:

  IgG2b

• Specificity:

  Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

• NCBI Gene Symbol:

  ITGAM

• Host or Source:

  Rat

• Gene Name URL:

  ITGAM

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000632