E2F1 Antibody

• Gene Name :

  E2F transcription factor 1

• Gene Aliases :

  RBP3, E2F-1, RBAP1, RBBP3

• Gene ID :

  1869

• Accession Number :

  NP_005216.1

• Reactivity :

  Human

• Immunogen :

  The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.

• Target :

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

• Clonality :

  Monoclonal

• Clone :

  10D1 2B5

• Type :

  Monoclonal Antibody

• Applications :

  ICC, IF, IP, WB

• Purification :

  Protein G purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Liquid

• Buffer :

  Phosphate-buffered saline, pH 7.4, containing no preservatives

• Reconstitution :

  Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

• Fragment :

  IgG2b

• NCBI Gene Symbol :

  E2F1

• Host or Source :

  Mouse

• Protein Name :

  Transcription factor E2F1

• Gene Name URL :

  E2F1

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_005225.2