IARS Antibody

• Gene Name:

  Isoleucyl-tRNA synthetase

• Gene Aliases:

  IRS, IARS, ILRS, ILERS, GRIDHH, PRO0785

• Gene ID:

  3376

• Accession Number:

  NP_002152.2

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human IARS. The exact sequence is proprietary.

• Target:

  Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  IHC-P, IP, WB

• Purification:

  Purified by antigen-affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.45 mg/ml

• Format:

  Liquid

• Buffer:

  1XPBS, 20% Glycerol (pH7) . 0.025% ProClin 300 was added as a preservative.

• Reconstitution:

  Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

• Fragment:

  IgG

• NCBI Gene Symbol:

  IARS

• Host or Source:

  Rabbit

• Protein Name:

  Isoleucine--tRNA ligase, cytoplasmic

• Gene Name URL:

  IARS

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_002161.5