U2AF2 Antibody

• Gene Name:

  U2 small nuclear RNA auxiliary factor 2

• Gene Aliases:

  U2AF65

• Gene ID:

  11338

• Accession Number:

  NP_001012496.1

• Reactivity:

  Human

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human U2AF65. The exact sequence is proprietary.

• Target:

  U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  ICC, IF, IHC-P, WB

• Purification:

  Purified by antigen-affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.79 mg/ml

• Format:

  Liquid

• Buffer:

  1XPBS, 1% BSA, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

• Reconstitution:

  Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

• Fragment:

  IgG

• NCBI Gene Symbol:

  U2AF2

• Host or Source:

  Rabbit

• Protein Name:

  Splicing factor U2AF 65 kDa subunit

• Gene Name URL:

  U2AF2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001012478.1