PSMD4 Antibody

• Gene Name:

  Proteasome 26S subunit, non-ATPase 4

• Gene Aliases:

  AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5

• Gene ID:

  5710

• Accession Number:

  NP_002801.1

• Reactivity:

  Human

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human PSMD4. The exact sequence is proprietary.

• Target:

  The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  ICC, IF, WB

• Purification:

  Purified by antigen-affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.93 mg/ml

• Format:

  Liquid

• Buffer:

  0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

• Reconstitution:

  Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

• Fragment:

  IgG

• NCBI Gene Symbol:

  PSMD4

• Host or Source:

  Rabbit

• Protein Name:

  26S proteasome non-ATPase regulatory subunit 4

• Gene Name URL:

  PSMD4

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_002810.2