MDM2 Antibody

• Gene Name:

  MDM2 proto-oncogene, E3 ubiquitin protein ligase

• Gene Aliases:

  HDMX, LSKB, hdm2, ACTFS

• Gene ID:

  4193

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.

• Target:

  This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  CHIP, IHC-FFPE, IP, WB

• Purification:

  Purified by antigen-affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1.2 mg/ml

• Homology:

  Rat (81%), Dog (91%), Cat (95%), Pig (93%), Rhesus Monkey (98%), Bovine (95%)

• Format:

  Liquid// 1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 as a preservative.

• Reconstitution:

  Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

• Molecular Weight:

  55 kDa

• Fragment:

  IgG

• NCBI Gene Symbol:

  MDM2

• Host or Source:

  Rabbit

• Gene Name URL:

  MDM2

• CAS Number:

  9007-83-4