CD86 Antibody

• Gene Name:

  CD86 antigen

• Gene Aliases:

  Activation B7-2 antigen; B7; B7.2; B70; B7-2; Cd28l; Cd28l2; CLS1; early T cell costimulatory molecule-1; Early T-cell costimulatory molecule 1; ETC-1; Ly5; Ly-5; Ly58; Ly-58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2.

• Gene ID:

  12524

• Accession Number:

  NP_001193853.1NP_001193854.1NP_008820.3NP_787058.4NP_795711.1

• Reactivity:

  Mouse

• Immunogen:

  LPS-activated CBA/Cs mouse splenic B cells

• Target:

  Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.

• Clonality:

  Monoclonal

• Clone:

  GL-1

• Conjugation:

  FITC

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry

• Purification:

  Purified by size-exclusion chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG2a

• Specificity:

  The rat monoclonal antibody GL-1 reacts with CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

• NCBI Gene Symbol:

  Cd86

• Host or Source:

  Rat

• Protein Name:

  T-lymphocyte activation antigen CD86

• Gene Name URL:

  Cd86

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001206924.1NM_001206925.1NM_006889.4NM_175862.4NM_176892.1