PDE8A Antibody

• Gene Name:

  Phosphodiesterase 8A

• Gene Aliases:

  CAMP-specific cyclic nucleotide phosphodiesterase 8A; high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; HsT19550.

• Gene ID:

  5151

• Accession Number:

  NP_001230066.1NP_002596.1NP_775656.1

• Reactivity:

  Human

• Immunogen:

  Human PDE8a

• Target:

  Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:18983167) . May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed:18983167) . Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed:23509299) . Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed:23509299) .

• Clonality:

  Monoclonal

• Clone:

  EM-52

• Type:

  Monoclonal Antibody

• Applications:

  Western blot

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG1

• Specificity:

  The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa enzyme that hydrolyzes cAMP to 5'AMP.

• NCBI Gene Symbol:

  PDE8A

• Host or Source:

  Mouse

• Protein Name:

  High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

• Gene Name URL:

  PDE8A

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001243137.1NM_002605.2NM_173454.1