TP53 Antibody

• Gene Name :

  Tumor protein p53

• Gene Aliases :

  Antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53.

• Gene ID :

  7157

• Accession Number :

  NP_000537.3NP_001119584.1NP_001119585.1NP_001119586.1NP_001119587.1NP_001119588.1

• Reactivity :

  Human|Non-human Primate

• Immunogen :

  Bacterially expressed full-length wild-type p53

• Target :

  Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492) .

• Clonality :

  Monoclonal

• Clone :

  BP53-12

• Type :

  Monoclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot

• Purification :

  Purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer :

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution :

  2°C to 8°C

• Fragment :

  IgG2a

• Specificity :

  The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.

• NCBI Gene Symbol :

  TP53

• Host or Source :

  Mouse

• Protein Name :

  Cellular tumor antigen p53

• Gene Name URL :

  TP53

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_000546.5NM_001126112.2NM_001126113.2NM_001126114.2NM_001126115.1NM_001126116.1