CDKN1A Antibody

• Gene Name:

  Cyclin dependent kinase inhibitor 1A

• Gene Aliases:

  CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CIP1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1) ; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; P21; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1.

• Gene ID:

  1026

• Accession Number:

  NP_000380.1NP_001207706.1NP_001207707.1NP_001278478.1NP_510867.1

• Reactivity:

  Human|Non-human Primate

• Immunogen:

  Human p21 protein

• Target:

  May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739) .

• Clonality:

  Monoclonal

• Clone:

  WA-1

• Type:

  Monoclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunohistochemistry-Paraffin

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG1

• Specificity:

  The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.

• NCBI Gene Symbol:

  CDKN1A

• Host or Source:

  Mouse

• Protein Name:

  Cyclin-dependent kinase inhibitor 1

• Gene Name URL:

  CDKN1A

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000389.4NM_001220777.1NM_001220778.1NM_001291549.1NM_078467.2