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NEFH Antibody

CAT: 0247-OAEE00502-100UGSize: 0.1 mg (C100)Dry Ice: NoHazardous: No
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CAT#:0247-OAEE00502-100UGSize:0.1 mg (C100)
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CAS Number
9007-83-4
Gene Name
Neurofilament heavy chain
Gene Aliases
200 kDa neurofilament protein; CMT2CC; neurofilament heavy polypeptide; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NFH; NF-H.
Gene ID
4744
Accession Number
NP_066554.2
Reactivity
Mammal
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Target
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Clonality
Monoclonal
Clone
NF-01
Type
Monoclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Reconstitution
2°C to 8°C
Fragment
IgG1
Specificity
The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
NCBI Gene Symbol
NEFH
Host or Source
Mouse
Protein Name
Neurofilament heavy polypeptide
Gene Name URL
NEFH
Nucleotide Accession Number
NM_021076.3

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