CD8A Antibody

• Gene Name:

  CD8 antigen, alpha chain

• Gene Aliases:

  BB154331; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100) ; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2.

• Gene ID:

  12525

• Accession Number:

  NP_001139345.1NP_001759.3NP_741969.1

• Reactivity:

  Mouse

• Immunogen:

  Murine spleen cells

• Target:

  Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs) . In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs) . This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

• Clonality:

  Monoclonal

• Clone:

  53-6.7

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry|Functional assay|Immunocytochemistry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4

• Buffer:

  Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG2a kappa

• Specificity:

  The rat monoclonal antibody 53-6.7 recognizes murine CD8a (32-34 kDa; alpha chain of the CD8 antigen) .

• NCBI Gene Symbol:

  Cd8a

• Host or Source:

  Rat

• Protein Name:

  T-cell surface glycoprotein CD8 alpha chain

• Gene Name URL:

  Cd8a

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001145873.1NM_001768.6NM_171827.3