Prkaca Antibody

• Gene Name:

  Protein kinase cAMP-activated catalytic subunit alpha|protein kinase cAMP-activated catalytic subunit beta|protein kinase cAMP-dependent type I regulatory subunit alpha|protein kinase cAMP-dependent type I regulatory subunit beta

• Gene Aliases:

  ACRDYS1; ADOHR; CAFD1; CAFD2; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; cAMP-dependent protein kinase type I-beta regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; cAMP-dependent protein kinase type II-beta regulatory chain; cAMP-dependent protein kinase type II-beta regulatory subunit; CAR; Carney complex type 1; CNC; CNC1; epididymis secretory sperm binding protein; H_RG363E19.2; PKA C-alpha; PKA C-beta; PKACA; PKACB; PKR1; PKR2; PPNAD1; PPNAD4; PRKAR1; PRKAR2; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase A type 1a regulatory subunit; protein kinase A, RII-alpha subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory subunit type I beta; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II beta; protein kinase, cAMP-dependent, regulatory, type I, alpha; protein kinase, cAMP-dependent, regulatory, type I, beta; protein kinase, cAMP-dependent, regulatory, type II, beta; RII-BETA; tissue-specific extinguisher 1; TSE1; WUGSC:H_RG363E19.2.

• Gene ID:

  5566|5567|5573|5575|5576|5577

• Accession Number:

  NP_001264827.1NP_032880.1

• Reactivity:

  Bovine|Dog|Guinea Pig|Hamster|Human|Monkey|Mouse|Porcine|Rabbit|Rat|Sheep|Xenopus

• Immunogen:

  Synthetic peptide corresponding to a portion of human PKA.

• Target:

  Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.|Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed:12420224, PubMed:21423175) . Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed:21880142) .|Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca (2+), leading to reduced amplitude and increased frequency of store overload-induced Ca (2+) release (SOICR) characterized by an increased rate of Ca (2+) release and propagation velocity of spontaneous Ca (2+) waves, despite reduced wave amplitude and resting cytosolic Ca (2+) . PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) . Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490) .|Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.|Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Western blot

• Purification:

  Protein A affinity purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid// PBS containing 50% glycerol and 0.09% sodium azide

• Reconstitution:

  Store long-term at -20° C. Avoid freeze/thaw cycles.

• Molecular Weight:

  ~40 kDa

• NCBI Gene Symbol:

  PRKACA|PRKACB|PRKAR1A|PRKAR1B|PRKAR2A|PRKAR2B

• Host or Source:

  Rabbit

• Protein Name:

  CAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit beta|cAMP-dependent protein kinase type I-alpha regulatory subunit|cAMP-dependent protein kinase type I-beta regulatory subunit|cAMP-dependent protein kinase type II-alpha regulatory subunit|cAMP-dependent protein kinase type II-beta regulatory subunit|Major capsid protein L1

• Gene Name URL:

  PRKACA|PRKACB|PRKAR1A|PRKAR1B|PRKAR2A|PRKAR2B

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001277898.1NM_008854.5