CD10 Antibody

• Gene Name:

  Membrane metallo-endopeptidase

• Gene Aliases:

  NEP, SFE, CD10, CALLA, CMT2T, SCA43

• Gene ID:

  4311

• Accession Number:

  NP_000893.2

• Reactivity:

  Human

• Target:

  This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL) . This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

• Clonality:

  Monoclonal

• Clone:

  B-E3

• Type:

  Monoclonal Antibody

• Applications:

  FC, IHC

• Purification:

  Ion exchange chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 ug/uL

• Bioactivity:

  Does not inhibit the Mixed Lymphocyte Culture, Cytotoxic in association with the Rabbit Complement on CD10+ Cells

• Format:

  Lyophilized

• Buffer:

  Phosphate-buffered saline. Sterile-filtered through 0.22 um and treated to remove endotoxins. Carrier and preservative free

• Reconstitution:

  Stable at +2-8C for 12 months. For longer storage, freeze aliquots

• Molecular Weight:

  86 kDa

• Fragment:

  IgG2a

• Specificity:

  Recognises the Common Acute Lymphoblastic Leukemia Antigen (CALLA), a 100 kDa protein

• NCBI Gene Symbol:

  CD10

• Host or Source:

  Mouse

• Protein Name:

  Neprilysin

• Gene Name URL:

  CD10

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000902.3