TERF2 Antibody

• Gene Name:

  Telomeric repeat binding factor 2

• Gene Aliases:

  Telomeric DNA-binding protein; telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TRBF2; TRF2; TTAGGG repeat-binding factor 2.

• Gene ID:

  7014

• Accession Number:

  NP_005643.2

• Reactivity:

  Human|Rat

• Immunogen:

  Range: Arg144 - Gln438

• Target:

  Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification:

  Affinity Purified; Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

• Reconstitution:

  2°C to 8°C|-20°C

• Molecular Weight:

  32 kDa

• Fragment:

  IgG

• Specificity:

  The antibody is a rabbit polyclonal antibody raised against TERF2. It has been selected for its ability to recognize TERF2 in immunohistochemical staining and western blotting.

• NCBI Gene Symbol:

  TERF2

• Host or Source:

  Rabbit

• Protein Name:

  Telomeric repeat-binding factor 2

• Gene Name URL:

  TERF2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_005652