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SHH Antibody

CAT: 0247-OACD07271-1MLSize: 1 mLDry Ice: NoHazardous: No
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CAT#:0247-OACD07271-1MLSize:1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Sonic hedgehog
Gene Aliases
9530036O11Rik; Dsh; hemimelic extra toes; Hhg1; HHG-1; Hx; Hxl3; M100081; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; short digits; sonic hedgehog protein.
Gene ID
20423
Accession Number
NP_033196.1
Reactivity
Human|Mouse|Rat
Immunogen
Recombinant SHH (Gly28~Ala193) expressed in E.coli
Target
Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed:8824192, PubMed:7891723) . Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed:8824192) . Both activities occur in the reticulum endoplasmic (PubMed:21357747) . Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed:21357747) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Source
Polyclonal antibody preparation
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.52 mg/ml
Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.
NCBI Gene Symbol
Shh
Host or Source
Rabbit
Protein Name
Sonic hedgehog protein
Gene Name URL
Shh
Nucleotide Accession Number
NM_009170

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