ERN1 Antibody

• Gene Name :

  Endoplasmic reticulum to nucleus signaling 1

• Gene Aliases :

  Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; inositol-requiring protein 1; IRE1; IRE1a; ire1-alpha; IRE1P; protein kinase/endoribonuclease; serine/threonine-protein kinase/endoribonuclease IRE1.

• Gene ID :

  2081

• Accession Number :

  NP_001424.3

• Reactivity :

  Human|Mouse|Rat

• Immunogen :

  Range: Phe571 - Phe832

• Target :

  Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875) . In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875) . Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875) . The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875) . The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875) .

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Immunohistochemistry|Western blot

• Purification :

  Affinity Purified; Protein A Purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  0.5 mg/ml

• Format :

  Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

• Reconstitution :

  2°C to 8°C|-20°C

• Molecular Weight :

  7 kDa

• Fragment :

  IgG

• Specificity :

  The antibody is a rabbit polyclonal antibody raised against ERN1. It has been selected for its ability to recognize ERN1 in immunohistochemical staining and western blotting.

• NCBI Gene Symbol :

  ERN1

• Host or Source :

  Rabbit

• Protein Name :

  Serine/threonine-protein kinase/endoribonuclease IRE1

• Gene Name URL :

  ERN1

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_001433