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DPPA3 Antibody

CAT: 0247-OACD00890-100ULSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0247-OACD00890-100ULSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Developmental pluripotency associated 3
Gene Aliases
Developmental pluripotency-associated protein 3; Pgc7; STELLA; stella-related protein.
Gene ID
359787
Accession Number
NP_954980.1
Reactivity
Human
Immunogen
Range: Met1 - Pro159
Target
Primordial germ cell (PGCs) -specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
18 kDa
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against DPPA3. It has been selected for its ability to recognize DPPA3 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
DPPA3
Host or Source
Rabbit
Protein Name
Developmental pluripotency-associated protein 3
Gene Name URL
DPPA3
Nucleotide Accession Number
NM_199286

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