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PUF60 Antibody

CAT: 0247-OACD00440-100ULSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0247-OACD00440-100ULSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Poly (U) binding splicing factor 60
Gene Aliases
60 kDa poly (U) -binding-splicing factor; FBP interacting repressor; FIR; FUSE-binding protein-interacting repressor; poly (U) binding splicing factor 60KDa; poly (U) -binding-splicing factor PUF60; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBPI; Siah binding protein 1; Siah-binding protein 1; SIAHBP1; VRJS.
Gene ID
22827
Accession Number
NP_001129505.1
Reactivity
Human|Mouse|Rat
Immunogen
Range: Asn226 - Gln549
Target
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly (U) RNA.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
54 kDa
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against PUF60. It has been selected for its ability to recognize PUF60 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
PUF60
Host or Source
Rabbit
Protein Name
Poly (U) -binding-splicing factor PUF60
Gene Name URL
PUF60
Nucleotide Accession Number
NM_001136033

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