CDYL Antibody

• Gene Name:

  Chromodomain Y like

• Gene Aliases:

  CDYL1; CDY-like, autosomal; chromodomain protein, Y-like; chromodomain Y-like protein; crotonyl-CoA hydratase; testis-specific chromodomain Y-like protein.

• Gene ID:

  9425

• Accession Number:

  NP_001137442

• Reactivity:

  Human

• Immunogen:

  Recombinant Human Chromodomain Y-like protein (8-260AA)

• Target:

  Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:19808672, PubMed:28402439) . Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:28402439) . Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2) ; acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (PubMed:22009739) . Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (PubMed:19061646) . Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (By similarity) . Required for neuronal migration during brain development by repressing expression of RHOA (By similarity) . In addition to act as a chromatin reader, acts as a hydro-lyase (PubMed:28803779) . Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E) -butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (PubMed:28803779) . Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (By similarity) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot

• Purification:

  Protein G Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid

• Reconstitution:

  -20°C or -80°C

• Fragment:

  IgG

• NCBI Gene Symbol:

  CDYL

• Host or Source:

  Rabbit

• Protein Name:

  Chromodomain Y-like protein

• Gene Name URL:

  CDYL

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001143970