PDHA1 Antibody

• Gene Name :

  Pyruvate dehydrogenase (lipoamide) alpha 1

• Gene Aliases :

  PDHA, PDHAD, PHE1A, PDHCE1A

• Gene ID :

  5160

• Swiss Prot :

  P08559

• Accession Number :

  NP_000275.1

• Reactivity :

  Human, Mouse

• Immunogen :

  Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1)

• Target :

  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Polyclonal Antibody

• Applications :

  WB, IF

• Purification :

  Affinity purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  Varies by lot. See vial for exact concentration.

• Format :

  Liquid.// PBS with 0.02% sodium azide, 50% glycerol, pH 7.3

• Reconstitution :

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight :

  47 kDa

• Fragment :

  IgG

• NCBI Gene Symbol :

  PDHA1

• Host or Source :

  Rabbit

• Protein Name :

  Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

• Gene Name URL :

  PDHA1

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_000284.3