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RFC5 Antibody

CAT: 0247-OAAL00280-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAL00280-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Replication factor C subunit 5
Gene Aliases
A1 36 kDa subunit; replication factor C (activator 1) 5, 36.5kDa; replication factor C subunit 5; replication factor C, 36-kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; RFC36.
Gene ID
5985
Accession Number
https://www.ncbi.nlm.nih.gov/protein/AAH13961
Reactivity
Human, Mouse
Immunogen
RFC5 (AAH13961, 1 a.a. ~ 340 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Target
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Clonality
Monoclonal
Clone
1B1
Type
Monoclonal Antibody
Sequence
METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINEDRLPHLLLYGPPGTGKTSTILACAKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMAPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALNILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDWMLNQDFTTAYRNITELKTLKGLALHDILTEIHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTNEKIQLSSLIAAFQVTRDLIVAEA
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid
Reconstitution
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
Fragment
IgG2a Kappa
NCBI Gene Symbol
RFC5
Host or Source
Mouse
Protein Name
Homo sapiens replication factor C (activator 1) 5, 36.5kDa, mRNA (cDNA clone MGC:4451 IMAGE:2960023), complete cds|Replication factor C (activator 1) 5, 36.5kDa [Homo sapiens]
Gene Name URL
RFC5
Nucleotide Accession Number
https://www.ncbi.nlm.nih.gov/nuccore/BC013961

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