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MCM3 Antibody

CAT: 0247-OAAL00189-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAL00189-100UGSize:100 µg
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CAS Number
9007-83-4
Gene Name
Minichromosome maintenance complex component 3
Gene Aliases
Cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3 minichromosome maintenance deficient 3; minichromosome maintenance deficient 3; P1.h; p102; P1-MCM3; replication licensing factor, beta subunit; RLF subunit beta; RLFB.
Gene ID
4172
Accession Number
https://www.ncbi.nlm.nih.gov/protein/NP_002379
Reactivity
Human, Mouse
Immunogen
MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Target
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq
Clonality
Monoclonal
Clone
2H3
Type
Monoclonal Antibody
Sequence
AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid.// PBS, pH 7.4
Reconstitution
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
Fragment
IgG2a Kappa
NCBI Gene Symbol
MCM3
Host or Source
Mouse
Protein Name
DNA replication licensing factor MCM3 isoform 1 [Homo sapiens]|Homo sapiens minichromosome maintenance complex component 3 (MCM3), transcript variant 1, mRNA
Gene Name URL
MCM3
Nucleotide Accession Number
https://www.ncbi.nlm.nih.gov/nuccore/NM_002388

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