CCND2 Antibody (Phospho-Thr280)

• Gene Aliases:

  MPPH3, KIAK0002

• Gene ID:

  894

• Swiss Prot:

  P30279

• Accession Number:

  NP_001750

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Cyclin D2 (Phospho-Thr280)

• Target:

  Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, ICC, IF, P-ELISA

• Purification:

  The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/mL

• Format:

  Liquid// PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol

• Reconstitution:

  Store at -20° C. Stable for 12 months from date of receipt.

• Molecular Weight:

  33 kDa

• Fragment:

  IgG

• Specificity:

  Cyclin D2 (Phospho-Thr280) Ab detects endogenous levels of total Cyclin D2 (Phospho-Thr280)

• NCBI Gene Symbol:

  CCND2

• Host or Source:

  Rabbit

• Protein Name:

  G1/S-specific cyclin-D2

• Gene Name URL:

  CCND2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001759