MID1 Antibody

• Gene Name:

  Midline 1

• Gene Aliases:

  OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, MIDIN, RNF59, ZNFXY, TRIM18

• Gene ID:

  4281

• Swiss Prot:

  O15344

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human TRI18

• Target:

  MID1 may have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. Belongs to the TRIM/RBCC family. A retroviral element acts as an alternative tissue- specific promoter for this gene. The LTR of an HERV-E element enhances the expression in placenta and embryonic kidney. Homodimer or heterodimer with MID2. Interacts with IGBP1. Two isoforms of the human protein are produced by alternative splicing.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Sequence:

  METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

• Applications:

  WB, IHC, IF, ICC

• Purification:

  Affinity-chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid.// PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Reconstitution:

  Store at -20C. Stable for 12 months from date of receipt.

• Molecular Weight:

  75 kDa

• Specificity:

  TRI18 antibody detects endogenous levels of total TRI18

• NCBI Gene Symbol:

  MID1

• Host or Source:

  Rabbit

• Gene Name URL:

  MID1

• CAS Number:

  9007-83-4