MDM2 Antibody

• Gene Name :

  MDM2 proto-oncogene, E3 ubiquitin protein ligase

• Gene Aliases :

  HDMX, LSKB, hdm2, ACTFS

• Gene ID :

  4193

• Swiss Prot :

  Q00987

• Reactivity :

  Human, Mouse

• Immunogen :

  A synthesized peptide derived from human MDM2

• Target :

  MDM2 a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  WB, IHC, IF, ICC

• Purification :

  Affinity-chromatography

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Liquid.// PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Reconstitution :

  Store at -20C. Stable for 12 months from date of receipt.

• Molecular Weight :

  90 kDa

• Specificity :

  MDM2 antibody detects endogenous levels of total MDM2

• NCBI Gene Symbol :

  MDM2

• Host or Source :

  Rabbit

• Gene Name URL :

  MDM2

• CAS Number :

  9007-83-4