EIF3F Antibody

• Gene Name :

  Eukaryotic translation initiation factor 3 subunit F

• Gene Aliases :

  Deubiquitinating enzyme eIF3f; eIF3 p47; eIF3-epsilon; eIF-3-epsilon; eIF3-p47; EIF3S5; Eukaryotic translation initiation factor 3 subunit 5; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) ; eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; MRT67.

• Gene ID :

  8665

• Swiss Prot :

  O00303

• Accession Number :

  NP_003745.1

• Reactivity :

  Human|Mouse|Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human EIF3F.

• Target :

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815) . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632) . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773) .

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution :

  The optimal working dilution should be determined by the end user.

• Format :

  Liquid

• Reconstitution :

  -20°C

• Molecular Weight :

  37 kDa

• Notes :

  Formerly GWB-ASE592

• Specificity :

  EIF3F antibody detects endogenous levels of total EIF3F protein.

• NCBI Gene Symbol :

  EIF3F

• Host or Source :

  Rabbit

• Protein Name :

  Eukaryotic translation initiation factor 3 subunit F

• Gene Name URL :

  EIF3F

• CAS Number :

  9007-83-4