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ATG4A Antibody

CAT: 0247-OAAF03545-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF03545-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Autophagy related 4A cysteine peptidase
Gene Aliases
APG4 autophagy 4 homolog A; APG4A; ATG4 autophagy related 4 homolog A; AUTL2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; autophagin 2; Autophagin-2; autophagy-related cysteine endopeptidase 2; autophagy-related protein 4 homolog A; cysteine protease ATG4A; hAPG4A.
Gene ID
115201
Swiss Prot
Q8WYN0
Accession Number
NP_443168.2
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human ATG4A.
Target
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
45 kDa
Notes
Formerly GWB-ASE512
Specificity
ATG4A antibody detects endogenous levels of total ATG4A protein.
NCBI Gene Symbol
ATG4A
Host or Source
Rabbit
Protein Name
Cysteine protease ATG4A
Gene Name URL
ATG4A

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