BATF Antibody

• CAS Number :

  9007-83-4

• Gene Name :

  Basic leucine zipper ATF-like transcription factor

• Gene Aliases :

  Activating transcription factor B; basic leucine zipper transcription factor, ATF-like; basic leucine zipper transcriptional factor ATF-like; B-ATF; BATF1; B-cell-activating transcription factor; SFA2; SFA-2; SF-HT-activated gene 2 protein.

• Gene ID :

  10538

• Swiss Prot :

  Q16520

• Accession Number :

  NP_006390.1

• Reactivity :

  Human|Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human BATF.

• Target :

  AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8 (+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B) . Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I (H) -C (H) ) . Following infection, can participate in CD8 (+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8 (+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs) : up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs (By similarity) .

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin

• Purification :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution :

  The optimal working dilution should be determined by the end user.

• Format :

  Liquid

• Reconstitution :

  -20°C

• Molecular Weight :

  14 kDa

• Notes :

  Formerly GWB-ASD699

• Specificity :

  BATF antibody detects endogenous levels of total BATF protein.

• NCBI Gene Symbol :

  BATF

• Host or Source :

  Rabbit

• Protein Name :

  Basic leucine zipper transcriptional factor ATF-like

• Gene Name URL :

  BATF