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BLM Antibody

CAT: 0247-OAAF00937-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF00937-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
BLM RecQ like helicase
Gene Aliases
Bloom syndrome protein; Bloom syndrome RecQ like helicase; Bloom syndrome, RecQ helicase-like; BS; DNA helicase, RecQ-like type 2; MGRISCE1; recQ protein-like 3; RECQ2; RECQL2; RECQL3.
Gene ID
641
Swiss Prot
P54132
Accession Number
NP_000048.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human Bloom Syndrome.
Target
ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9388193, PubMed:24816114, PubMed:25901030) . Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288) . Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134) . Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030) . Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030) . Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (PubMed:20639533, PubMed:24257077, PubMed:25901030) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
159 kDa
Notes
Formerly GWB-ASC350
Specificity
Bloom Syndrome antibody detects endogenous levels of total Bloom Syndrome protein.
NCBI Gene Symbol
BLM
Host or Source
Rabbit
Protein Name
Bloom syndrome protein
Gene Name URL
BLM

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