TP53BP1 Antibody

• Description:

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• Gene Name:

  Tumor protein p53 binding protein 1

• Gene Aliases:

  53BP1; p202; p53-binding protein 1; p53BP1; TDRD30; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1.

• Gene ID:

  7158

• Swiss Prot:

  Q12888

• Accession Number:

  NP_001135451.1

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human 53BP1.

• Target:

  Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136) . Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ) -mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306) . In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136) . Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600) . Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425) . Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity) . In contrast, it is not required for classic NHEJ and V (D) J recombination (By similarity) . Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  213 kDa

• Notes:

  Formerly GWB-ASC335

• Specificity:

  53BP1 (Ab-6) antibody detects endogenous levels of total 53BP1 protein

• NCBI Gene Symbol:

  TP53BP1

• Host or Source:

  Rabbit

• Protein Name:

  TP53-binding protein 1

• Gene Name URL:

  TP53BP1

• CAS Number:

  9007-83-4