TSC2 Antibody

• Description:

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• Gene Name:

  TSC complex subunit 2

• Gene Aliases:

  LAM; PPP1R160; protein phosphatase 1, regulatory subunit 160; TSC4; tuberin; tuberous sclerosis 2 protein.

• Gene ID:

  7249

• Swiss Prot:

  P49815

• Accession Number:

  NP_000539.2

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human Tuberin/TSC2.

• Target:

  In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400) . Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:15340059) . May also play a role in microtubule-mediated protein transport (By similarity) . Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid// PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+

• Reconstitution:

  -20°C

• Molecular Weight:

  200 kDa

• Notes:

  Formerly GWB-ASC265

• Fragment:

  IgG

• Specificity:

  Tuberin/TSC2 Antibody detects endogenous levels of total Tuberin/TSC2 protein.

• NCBI Gene Symbol:

  TSC2

• Host or Source:

  Rabbit

• Protein Name:

  Tuberin

• Gene Name URL:

  TSC2

• CAS Number:

  9007-83-4