RUNX1 Antibody

• Gene Name:

  RUNX family transcription factor 1

• Gene Aliases:

  Acute myeloid leukemia 1 protein; AML1; AML1-ETO fusion; AML1-ETO fusion protein; AML1-EVI-1; AML1-EVI-1 fusion protein; AMLCR1; CBF2alpha; CBFA2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; EVI-1; mutant RUNX1; oncogene AML-1; PEA2-alpha B; PEBP2aB; PEBP2alpha; PEBP2-alpha B; polyomavirus enhancer-binding protein 2 alpha B subunit; runt related transcription factor 1; runt-related transcription factor 1; SL3/AKV core-binding factor alpha B subunit; SL3-3 enhancer factor 1 alpha B subunit.

• Gene ID:

  861

• Swiss Prot:

  Q01196

• Accession Number:

  NP_001001890.1

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human AML1.

• Target:

  Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable) . Essential for the development of normal hematopoiesis (PubMed:17431401) . Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218) . Inhibits KAT6B-dependent transcriptional activation (By similarity) . Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity) . Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532) . Positively regulates the expression of RORC in T-helper 17 cells (By similarity) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

• Reconstitution:

  -20°C

• Molecular Weight:

  48 kDa

• Notes:

  Formerly GWB-ASC122

• Fragment:

  IgG

• Specificity:

  AML1 Antibody detects endogenous levels of total AML1 protein

• NCBI Gene Symbol:

  RUNX1

• Host or Source:

  Rabbit

• Protein Name:

  Runt-related transcription factor 1

• Gene Name URL:

  RUNX1

• CAS Number:

  9007-83-4