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ACO1 Antibody (Phospho-Ser711)

CAT: 0247-OAAF00239-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF00239-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Aconitase 1
Gene Aliases
Aconitase 1, soluble; aconitate hydratase, cytoplasmic; ACONS; citrate hydro-lyase; cytoplasmic aconitase; cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; epididymis secretory sperm binding protein; ferritin repressor protein; HEL60; IREB1; IREBP; IREBP1; iron regulatory protein 1; iron-responsive element-binding protein 1; IRP1; soluble aconitase.
Gene ID
48
Swiss Prot
P21399
Accession Number
NP_002188.1
Reactivity
Human|Monkey|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711.
Target
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
98 kDa
Notes
Formerly GWB-ASB505
Specificity
IREB1 (Phospho-Ser711) antibody detects endogenous levels of IREB1 only when phosphorylated at serine 711.
NCBI Gene Symbol
ACO1
Host or Source
Rabbit
Protein Name
Cytoplasmic aconitate hydratase
Gene Name URL
ACO1

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