JUN Antibody (Phospho-Thr231)

• Gene Name:

  Jun proto-oncogene, AP-1 transcription factor subunit

• Gene Aliases:

  Activator protein 1; AP1; AP-1; c-Jun; cJUN; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; p39; proto-oncogene cJun; proto-oncogene c-Jun; transcription factor AP-1; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog.

• Gene ID:

  3725

• Swiss Prot:

  P05412

• Accession Number:

  NP_002219.1

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Thr231.

• Target:

  Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306) . Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  35 kDa

• Notes:

  Formerly GWB-ASB389

• Specificity:

  C-Jun (Phospho-Thr231) antibody detects endogenous levels of c-Jun only when phosphorylated at threonine 231.

• NCBI Gene Symbol:

  JUN

• Host or Source:

  Rabbit

• Protein Name:

  Transcription factor AP-1

• Gene Name URL:

  JUN

• CAS Number:

  9007-83-4