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JUN Antibody (Phospho-Thr231)

CAT: 0247-OAAF00184-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF00184-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Jun proto-oncogene, AP-1 transcription factor subunit
Gene Aliases
Activator protein 1; AP1; AP-1; c-Jun; cJUN; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; p39; proto-oncogene cJun; proto-oncogene c-Jun; transcription factor AP-1; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog.
Gene ID
3725
Swiss Prot
P05412
Accession Number
NP_002219.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Thr231.
Target
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306) . Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
35 kDa
Notes
Formerly GWB-ASB389
Specificity
C-Jun (Phospho-Thr231) antibody detects endogenous levels of c-Jun only when phosphorylated at threonine 231.
NCBI Gene Symbol
JUN
Host or Source
Rabbit
Protein Name
Transcription factor AP-1
Gene Name URL
JUN

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