AP2A2 Antibody

• Gene Name:

  AP-2 complex subunit alpha-2

• Gene Aliases:

  HIP9, HYPJ, ADTAB, HIP-9, CLAPA2

• Gene ID:

  161

• Swiss Prot:

  O94973

• Accession Number:

  NP_001229766.1NP_036437.1

• Reactivity:

  Human, Rat

• Immunogen:

  AP-2 adaptor polypeptides from bovine brain.

• Target:

  Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. . Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

• Clonality:

  Monoclonal

• Clone:

  Ada-1

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Purification:

  Purified by the goat anti-mouse IgG Affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Buffer:

  1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

• Reconstitution:

  At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.

• Fragment:

  IgG2a

• NCBI Gene Symbol:

  AP2A2

• Host or Source:

  Mouse

• Protein Name:

  AP-2 complex subunit alpha-2

• Gene Name URL:

  AP2A2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001242837.1NM_012305.3