CD22 Antibody

• Gene Aliases:

  SIGLEC2, SIGLEC-2

• Gene ID:

  933

• Swiss Prot:

  P20273

• Reactivity:

  Human

• Immunogen:

  Raji (Burkitt's lymphoma) cells.

• Target:

  Monoclonal Mouse Anti-Human CD22

• Clonality:

  Monoclonal

• Clone:

  B456 (FR10B4)

• Type:

  Monoclonal Antibody

• Applications:

  IHC-F, FC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid.// 20mM Tris-borate, 150mM NaCl, RPMI 1640/D-MEM contaiing fetal bovine serum, GMC-6 carrier polysaccharides, carrier protein and 0.05% sodium azide, pH 7.5

• Reconstitution:

  Store at 2 - 8C for short term, freeze under -20C for long term storage.

• Fragment:

  IgG1

• Specificity:

  This antibody reacts strongly with CD22. CD22 is expressed on in all B cell cytoplasm. It appears as CD19 on the cell surface in developing B cells. It can also be found on the cell surface of most mature sIg+ B cells and on hairy cell and prolymphocytic leukemia cells. On frozen sections, CD22 is found in follicular mantle and marginal zone B cells. There is weak reaction in germinal center B cells. This antibody can be used for typing of leukemia, assigning B cell lineage, and in flow cytometry to identify mature B cells. Also, it can be used to study B cell adhesion and to identify B cell lymphomas.

• NCBI Gene Symbol:

  CD22

• Host or Source:

  Mouse

• Protein Name:

  B-cell receptor CD22

• Gene Name URL:

  CD22

• CAS Number:

  9007-83-4