B CELL ANTIGEN Antibody

• Reactivity:

  Human

• Immunogen:

  Human B cell line Daudi.

• Target:

  Monoclonal Mouse Anti-Human B Cell, B cell Antigen

• Clonality:

  Monoclonal

• Clone:

  B381 (IPO10)

• Type:

  Monoclonal Antibody

• Applications:

  WB, IHC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Concentrated

• Reconstitution:

  Store at 2 - 8C for short term, freeze under -20C for long term storage.

• Fragment:

  IgG3

• Specificity:

  This antibody defines the antigen which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD 37 and cym. It is expressed on resting B cells and then reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. It is a useful antibody for studying B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells) . This antibody does not label neoplasms of T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4