GAPDH Antibody

• Gene Name:

  Glyceraldehyde-3-phosphate dehydrogenase

• Gene Aliases:

  G3PD, GAPD, HEL-S-162eP

• Gene ID:

  2597

• Swiss Prot:

  P04406

• Accession Number:

  NP_001243728.1

• Reactivity:

  Human, Mouse

• Immunogen:

  This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH.

• Target:

  This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

• Clonality:

  Monoclonal

• Clone:

  1A10A10

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Purification:

  Protein G column purification, followed by dialysis against PBS

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  310 ug/mL

• Homology:

  Human, Mouse

• Format:

  Liquid

• Reconstitution:

  Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  36 kDa

• Fragment:

  IgG1, kappa

• NCBI Gene Symbol:

  GAPDH

• Host or Source:

  Mouse

• Gene Name URL:

  GAPDH

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001256799.2