USP5 Antibody

• Gene Aliases:

  ISOT

• Gene ID:

  8078

• Swiss Prot:

  P45974

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.

• Target:

  Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Purification:

  This antibody is purified through a protein G column, followed by dialysis against PBS.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Reconstitution:

  Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  96 kDa

• Fragment:

  IgG1, kappa

• NCBI Gene Symbol:

  USP5

• Host or Source:

  Mouse

• Gene Name URL:

  USP5

• CAS Number:

  9007-83-4