MGMT Antibody

• Gene Aliases:

  MGMT, Methylated-DNA--protein-cysteine methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

• Gene ID:

  4255

• Swiss Prot:

  P16455

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged MGMT protein was used to produced this monoclonal antibody.

• Target:

  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Purification:

  This antibody is purified through a protein G column, followed by dialysis against PBS.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Reconstitution:

  Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  22 kDa

• Fragment:

  IgG1

• NCBI Gene Symbol:

  MGMT

• Host or Source:

  Mouse

• Gene Name URL:

  MGMT

• CAS Number:

  9007-83-4