H3F3A

• Gene Name:

  Histone cluster 3, H3

• Gene Aliases:

  H3t, H3.4, H3/g, H3FT, HIST3H3

• Gene ID:

  8290

• Swiss Prot:

  P84243

• Accession Number:

  NP_003484

• Reactivity:

  Human

• Immunogen:

  The immunogen is a synthetic peptide directed towards the N terminal region of human HIST3H3

• Target:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

• Partner Proteins:

  RAD6; SETD7; WHSC1L1; PRDM2; WHSC1; KMT2A; PHF7; CBX5; TRIM28; ING2; UBC; K07C5.3; APLF; UHRF1; NEDD8; CUL4A; EZH2; ASF1B; ASF1A; MMS22L; IGSF8; TONSL; ICAM1; CD81; AURKA; MLLT1; TAF8; TAF3; TAF9B; PHF21A; ING4; PRAME; KAT6B; CBX6; PHF8; PHF2; ITGA4; TAF1

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Sequence:

  MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALR

• Applications:

  WB

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Reconstitution:

  For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  15kDa

• Protein Length:

  136

• NCBI Gene Symbol:

  HIST3H3

• Host or Source:

  Rabbit

• Protein Name:

  Histone H3.3

• Gene Name URL:

  HIST3H3

• Nucleotide Accession Number:

  NM_003493