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Collagen Type I, mouse Antibody

CAT: 0013-GTR17702219Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR17702219Size:0.1 mL
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Description
Rabbit polyclonal antibody to Collagen Type I
UniProt
P11087
Immunogen
Purified collagen type I from murine skin
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration
App. 1 mg/ml
Dilution
(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:500, ELISA ? 1:200 (OD ? 500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Mouse skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC-P, RIA, WB
Host or Source
Rabbit

UniProtKB · P11087

Collagen alpha-1(I) chain

CO1A1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P11087
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Col1a1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q53WT0, Q60635, Q61367, Q61427, Q63919, Q6PCL3, Q810J9
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Domain

CollagenRepeatSignal

PTM

Disulfide bondGlycoproteinHydroxylationPhosphoprotein

Cellular component

Extracellular matrixSecreted

Technical term

Reference proteome

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