Rat SALb (Salusin Beta) ELISA Kit

Rat SALb (Salusin Beta) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SALb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SALb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SALb in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Salusin Beta
• Field of Research: Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hormone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 10 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.041 ng/mL