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Elastin, human Antibody

CAT: 0013-GTR17702210Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR17702210Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Guinea pig polyclonal antibody to Elastin, human
UniProt
P15502
Immunogen
Purified elastin from human skin
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration
App. 1 mg/ml
Dilution
(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:500 - 1:1000, ELISA ? 1:200 (OD ? 500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Human skin or aorta. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC-P, RIA
Host or Source
Guinea pig

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