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Fibronectin, rat Antibody

CAT: 0013-GTR17702209Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR17702209Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rabbit polyclonal antibody to Fibronectin
UniProt
P04937
Immunogen
Purified fibronectin from rat plasma
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile) . Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent.
Concentration
App. 1 mg/ml
Dilution
(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:1000, ELISA ? 1:200 (OD ? 500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Rat skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC-P, RIA, WB
Host or Source
Rabbit

UniProtKB · P04937

Fibronectin

FINC_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P04937
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Fn1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
FN
EC number
—
Processing
Precursor
Secondary accessions
Q6LDX9
Protein keywords

Biological process

Acute phaseAngiogenesisCell adhesionCell shape

Coding sequence diversity

Alternative splicing

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinIsopeptide bondOxidationPhosphoproteinSulfation

Cellular component

Extracellular matrixSecreted

Molecular function

Heparin-binding

Domain

RepeatSignal

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