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Alpha-amylase Antibody

CAT: 0013-GTR18577304Size: 50 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18577304Size:50 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Alpha-amylase Antibody
Product Name Alternative
Rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase
UniProt
P00692
Reactivity
Bacteria
Immunogen
A-Amylase [Bacillus amyloliquefaciens]
Target
Alpha-amylase
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
10.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against a-Amylase from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 ug of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 5.0 mL
Tested Applications
ELISA, IP, WB
NCBI Accession Number
WP_013352208.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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